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ZHOU Yongqing, WANG Jianghe, LIN Hengyan, YANG Kaiqi, RAO Nan, WANG Man, CHU Hongjuan. Application value of dual amplification method for nucleic acid detection of seven respiratory pathogens by throat swab samples in diagnosis of acute upper respiratory tract infections in children[J]. Journal of Clinical Medicine in Practice, 2025, 29(3): 114-117. DOI: 10.7619/jcmp.20240803
Citation: ZHOU Yongqing, WANG Jianghe, LIN Hengyan, YANG Kaiqi, RAO Nan, WANG Man, CHU Hongjuan. Application value of dual amplification method for nucleic acid detection of seven respiratory pathogens by throat swab samples in diagnosis of acute upper respiratory tract infections in children[J]. Journal of Clinical Medicine in Practice, 2025, 29(3): 114-117. DOI: 10.7619/jcmp.20240803
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Application value of dual amplification method for nucleic acid detection of seven respiratory pathogens by throat swab samples in diagnosis of acute upper respiratory tract infections in children

  • Department of Pathology, Hainan Hospital of Shanghai Children's Medical Center Affiliated to Medical College of Shanghai Jiaotong University, Sanya, Hainan, 572000

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  • Received Date: February 27, 2024
  • Revised Date: June 10, 2024
    Graphical Abstract
    • Abstract
  • Objective 

    To compare the diagnostic values of different detection methods for respiratory pathogens in children with acute respiratory infections.

    Methods 

    A total of 862 children with acute respiratory infections were enrolled, and their throat swab samples were tested for seven common respiratory pathogens by the dual amplification method and a self-built nucleic acid detection system. For samples with inconsistent results between the two methods, nested polymerase chain reaction (PCR) was performed for verification.

    Results 

    The positive detection rate of the dual amplification method was 57.75%, which was significantly higher than 30.14% of the self-built nucleic acid detection system, and the detection rate of mixed infections was 10.14%, which was also significantly higher than 1.97% of the self-built nucleic acid detection system (P < 0.05). The sensitivity of the dual amplification method was 91.63%, which was significantly higher than 72.61% of the self-built nucleic acid detection system, and the specificity was 92.31%, which was also significantly higher than 75.62% of the self-built nucleic acid detection system (P < 0.05).

    Conclusion 

    The dual amplification method can simultaneously detect the ribonucleic acid of seven respiratory pathogens with high sensitivity and specificity, demonstrating significant clinical application value.

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    • References (21)
  • [1]
    LEVINE S M, MARCINIUK D D. Global impact of respiratory disease: what can we do, together, to make a difference[J]. Chest, 2022, 161(5): 1153-1154. doi: 10.1016/j.chest.2022.01.014
    [2]
    MURGIA V, MANTI S, LICARI A, et al. Upper respiratory tract infection-associated acute cough and the urge to cough: new insights for clinical practice[J]. Pediatr Allergy Immunol Pulmonol, 2020, 33(1): 3-11. doi: 10.1089/ped.2019.1135
    [3]
    WANG D Y, ECCLES R, BELL J, et al. Management of acute upper respiratory tract infection: the role of early intervention[J]. Expert Rev Respir Med, 2021, 15(12): 1517-1523. doi: 10.1080/17476348.2021.1988569
    [4]
    LIN C Y, HWANG D, CHIU N C, et al. Increased detection of viruses in children with respiratory tract infection using PCR[J]. Int J Environ Res Public Health, 2020, 17(2): 564. doi: 10.3390/ijerph17020564
    [5]
    徐正, 何成山, 任书文, 等. 双扩增法检测呼吸道病原体RNA的性能评价[J]. 中国医药, 2021, 16(11): 1669-1673.
    [6]
    王天有, 申昆玲, 沈颖. 诸福棠实用儿科学[M]. 9版. 北京: 人民卫生出版社, 2022: 1-12.
    [7]
    CILLÓNIZ C, PERICÀS J M, ROJAS J R, et al. Severe infections due to respiratory viruses[J]. Semin Respir Crit Care Med, 2022, 43(1): 60-74. doi: 10.1055/s-0041-1740982
    [8]
    KRILOV L R, ROBERTS N J Jr. Respiratory syncytial virus (RSV) update[J]. Viruses, 2022, 14(10): 2110. doi: 10.3390/v14102110
    [9]
    ZHANG Y W, YUAN L C, ZHANG Y M, et al. Burden of respiratory syncytial virus infections in China: Systematic review and meta-analysis[J]. J Glob Health, 2015, 5(2): 020417. doi: 10.7189/jogh.05.020417
    [10]
    DABANIYASTI D, EKSI F, KESKIN Ö, et al. An investigation into respiratory tract viruses in children with acute lower respiratory tract infection or wheezing[J]. Minerva Pediatr, 2020, 72(1): 45-54. http://pubmed.ncbi.nlm.nih.gov/27854115/
    [11]
    PEARSON K, DOHERTY C, ZHANG D, et al. Optimized quantitative PCR analysis of random DNA aptamer libraries[J]. Anal Biochem, 2022, 650: 114712. doi: 10.1016/j.ab.2022.114712
    [12]
    王伟, 沈国理, 陈启荣, 等. 实时荧光PCR法快速检测急性上呼吸道感染病原体[J]. 武警医学院学报, 2009, 18(12): 1022-1024.
    [13]
    WU S W, ABDULLAH AL-MASKRI A A, LI Q, et al. A novel miRNA detection method using loop-mediated isothermal amplification[J]. Int J Anal Chem, 2023, 2023: 6624884. http://openurl.ebsco.com/contentitem/doi:10.1155%2F2023%2F6624884?sid=ebsco:plink:crawler&id=ebsco:doi:10.1155%2F2023%2F6624884
    [14]
    LIU S J, WENG B R, LIU Y Q, et al. Dual-signal cascaded nucleic acid amplification circuit-loaded metal-organic frameworks for accurate and robust imaging of intracellular microRNA[J]. Langmuir, 2023, 39(30): 10453-10463. doi: 10.1021/acs.langmuir.3c00897
    [15]
    罗金秀, 邹海珊, 祝少凤, 等. 三项呼吸道病原体核酸检测双扩增法在患儿呼吸道疾病诊断的临床价值[J]. 医学检验与临床, 2019, 30(6): 5-8.
    [16]
    LIU C, WEI X Y, ZHANG H M, et al. Nucleic acid hybridization enhanced luminescence for rapid and sensitive RNA and DNA based diagnostics[J]. Anal Chem, 2022, 94(46): 15964-15970. doi: 10.1021/acs.analchem.2c02673
    [17]
    李因美. 呼吸道病原菌核酸检测对下呼吸道感染诊断中的应用价值[J]. 保健文汇, 2023, 24(17): 69-72.
    [18]
    DIMECH W. The standardization and control of serology and nucleic acid testing for infectious diseases[J]. Clin Microbiol Rev, 2021, 34(4): e0003521. doi: 10.1128/CMR.00035-21
    [19]
    WENG Z, YU H Y, LUO W, et al. Specific and robust hybridization based on double-stranded nucleic acids with single-base resolution[J]. Anal Chim Acta, 2022, 1199: 339568. doi: 10.1016/j.aca.2022.339568
    [20]
    黄小兰, 贾楠, 肖飞, 等. 呼吸道病原体核酸双扩增法在儿童上呼吸道感染病原诊断中的应用研究[J]. 中华实验和临床病毒学杂志, 2020, 34(1): 57-60.
    [21]
    LI Z J, ZHANG H Y, REN L L, et al. Etiological and epidemiological features of acute respiratory infections in China[J]. Nat Commun, 2021, 12(1): 5026. doi: 10.1038/s41467-021-25120-6
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