Effect of resveratrol on endoplasmic reticulum stress and apoptosis in thyroid follicular cells induced by high iodine

ZHANG Yifei; LI Shuqin; YANG Zhiyong

doi:10.7619/jcmp.20240433

Journal of Clinical Medicine in Practice > 2024 > 28(16): 44-48. > DOI: 10.7619/jcmp.20240433

ZHANG Yifei, LI Shuqin, YANG Zhiyong. Effect of resveratrol on endoplasmic reticulum stress and apoptosis in thyroid follicular cells induced by high iodine[J]. Journal of Clinical Medicine in Practice, 2024, 28(16): 44-48. DOI: 10.7619/jcmp.20240433

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Effect of resveratrol on endoplasmic reticulum stress and apoptosis in thyroid follicular cells induced by high iodine

1.
Department of Oncology, Chongming Hospital Affiliated to Shanghai College of Health Medicine, Shanghai, 202150
2.
Department of Hematology, Chongming Hospital Affiliated to Shanghai College of Health Medicine, Shanghai, 202150

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Received Date: January 23, 2024
Revised Date: March 12, 2024

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Abstract

Abstract

Objective
To investigate the effect of resveratrol on endoplasmic reticulum stress and apoptosis in thyroid follicular cells induced by high iodine.
Methods
The thyroid follicular cell line Nthy-ori 3-1 was cultured in vitro and divided into four groups based on different culture methods: normal culture group (Normal group), high iodine group (HI group), resveratrol group (RES group), and high iodine combined with resveratrol group (HI+RES group). Cell proliferation activity was detected by CCK-8 assay, cell apoptosis was detected by TUNEL assay, and the BIP, CHOP, IRE1 mRNA expressions were detected by real-time quantitative polymerase chain reaction (PCR). Western blot was used to detect the protein expressions of BIP, CHOP, and IRE1.
Results
Compared with the Normal group, the proliferation activity of cells in the HI group decreased after 72 hours of culture, the proportion of apoptotic cells increased, the proportion of TUNEL staining positive cells increased, and the relative expressions of BIP, CHOP, IRE1 mRNA, as well as BIP and CHOP proteins were significantly elevated (P < 0.001 or P < 0.05). Compared with the HI group, the proliferation activity of cells in the HI+RES group increased after 72 hours of culture, the proportion of apoptotic cells decreased, the proportion of TUNEL staining positive cells decreased, and the relative expressions of BIP, CHOP mRNA, as well as BIP and CHOP proteins, were significantly reduced(P < 0.001 or P < 0.01).
Conclusion
Resveratrol exerts a protective effect on thyroid follicular cells induced by high iodine, significantly inhibiting endoplasmic reticulum stress and apoptosis caused by excessive iodine intake.
- resveratrol,
- thyroid follicular cells,
- iodine excess,
- endoplasmic reticulum stress,
- apoptosis

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References

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