LIN Jiejin, HE Qing, ZHANG Junfeng, FAN Yuqi. Role of telomeric repeat-binding factor 2-P53 signal pathway in diabetic cardiomyopathy[J]. Journal of Clinical Medicine in Practice, 2023, 27(21): 59-63, 100. DOI: 10.7619/jcmp.20232199
Citation: LIN Jiejin, HE Qing, ZHANG Junfeng, FAN Yuqi. Role of telomeric repeat-binding factor 2-P53 signal pathway in diabetic cardiomyopathy[J]. Journal of Clinical Medicine in Practice, 2023, 27(21): 59-63, 100. DOI: 10.7619/jcmp.20232199

Role of telomeric repeat-binding factor 2-P53 signal pathway in diabetic cardiomyopathy

More Information
  • Received Date: July 10, 2023
  • Revised Date: September 17, 2023
  • Available Online: November 23, 2023
  • Objective 

    To study the role of telomeric repeat-binding factor 2-P53(TRF2-P53) signal pathway in diabetic cardiomyopathy.

    Methods 

    In vivo study, type 1 diabetes mouse model was induced by intraperitoneal injection of streptozocin(STZ). After 9 weeks, DCM model was successfully established. The expression level of TRF2 in mouse heart samples was detected by Western blot (WB) and P53 by immunofluorescence. In vitro studies, high sugar was given to stimulate the cardiac muscle cells of neonatal mice, TRF2 expression level was detected by WB and P53 expression level was detected by immunofluorescence. TRF2 siRNA was used to reduce the expression of TRF2 in neonatal mice cardiomyocytes, then TUNEL was used to detect apoptosis, SA-β-galactosidase (SA-β-gal) staining was used to detect condition of aging, and P53 expression level was detected by WB.

    Result 

    In vivo studies found that TRF2 expression decreased and P53 expression increased in DCM mice compared with WT mice. In vitro studies showed that high glucose stimulation caused the decrease of TRF2 expression and the increase of P53 expression in cardiomyocytes of lactating rat. TRF2 siRNA transfection can promote apoptosis and senescence and increase P53 expression after reducing TRF2 level in cardiomyocytes of lactating rat.

    Conclusion 

    TRF2-P53 signaling pathway plays an important role in the regulation of DCM.

  • [1]
    黄翠华, 林露, 黄丽珊, 等. 线粒体自噬在糖尿病心肌病中的研究进展[J]. 中华内分泌代谢杂志, 2023, 39(6): 522-526. doi: 10.3760/cma.j.cn311282-20221028-00596
    [2]
    MURTAZA G, VIRK H U H, KHALID M, et al. Diabetic cardiomyopathy - A comprehensive updated review[J]. Prog Cardiovasc Dis, 2019, 62(4): 315-326. doi: 10.1016/j.pcad.2019.03.003
    [3]
    张晓天, 亢梦婕, 董嘉薇, 等. 钠钾泵抑制剂pNaKtide对糖尿病心肌病小鼠心脏损伤的作用[J]. 郑州大学学报: 医学版, 2023, 58(2): 153-157. https://www.cnki.com.cn/Article/CJFDTOTAL-HNYK202302002.htm
    [4]
    张驰, 黄静, 杨萍, 等. 鸢尾素通过调控NF-κB通路减轻糖尿病心肌病炎症反应[J]. 四川大学学报: 医学版, 2023, 54(3): 545-551. https://www.cnki.com.cn/Article/CJFDTOTAL-HXYK202303015.htm
    [5]
    LANGE T D. How telomeres solve the end-protection problem[J]. Science, 2009, 326(5955): 948-952. doi: 10.1126/science.1170633
    [6]
    IMRAN S A M, YAZID M D, CUI W, et al. The intra- and extra-telomeric role of TRF2 in the DNA damage response[J]. Int J Mol Sci, 2021, 22(18): 9900. doi: 10.3390/ijms22189900
    [7]
    TIMASHEV L A, LANGE T D. Characterization of t-loop formation by TRF2[J]. Nucleus, 2020, 11(1): 164-177. doi: 10.1080/19491034.2020.1783782
    [8]
    EGUCHI A, GONZALEZ A F G S, TORRES-BIGIO S I, et al. TRF2 rescues telomere attrition and prolongs cell survival in Duchenne muscular dystrophy cardiomyocytes derived fromhuman iPSCs[J]. Proc Natl Acad Sci U S A, 2023, 120(6): e2209967120. doi: 10.1073/pnas.2209967120
    [9]
    DILLMANN W H. Diabetic cardiomyopathy[J]. Circ Res, 2019, 124(8): 1160-1162. doi: 10.1161/CIRCRESAHA.118.314665
    [10]
    周乐群, 唐熠达. 糖尿病心肌病的发病机制及胰高血糖素样肽1受体激动剂对其影响[J]. 中华心血管病杂志, 2023, 51(4): 440-442.
    [11]
    杨嫒萍, 张磊, 李炜, 等. 黄芪甲苷对糖尿病心肌病小鼠的治疗作用及其机制[J]. 山东医药, 2022, 62(23): 19-24. https://www.cnki.com.cn/Article/CJFDTOTAL-SDYY202223005.htm
    [12]
    LORENZO-ALMORÓS A, CEPEDA-RODRIGO J M, LORENZO Ó. Diabetic cardiomyopathy[J]. Rev Clin Esp, 2022, 222(2): 100-111. doi: 10.1016/j.rce.2019.10.013
    [13]
    KARLSEDER J, BROCCOLI D, DAI Y, et al. p53- and ATM-dependent apoptosis induced by telomeres lacking TRF2[J]. Science, 1999, 283(5406): 1321-1325. doi: 10.1126/science.283.5406.1321
    [14]
    MARTÍNEZ P, FERRARA-ROMEO I, FLORES J M, et al. Essential role for the TRF2 telomere protein in adult skin homeostasis[J]. Aging Cell, 2014, 13(4): 656-668. doi: 10.1111/acel.12221
    [15]
    STAGNO D'ALCONTRES M, MENDEZ-BERMUDEZ A, FOXON J L, et al. Lack of TRF2 in ALT cells causes PML-dependent p53 activation and loss of telomeric DNA[J]. J Cell Biol, 2007, 179(5): 855-867. doi: 10.1083/jcb.200703020
    [16]
    YANG Q H, NIE Z Y, ZHU Y K, et al. Inhibition of TRF2 leads to ferroptosis, autophagic death, and apoptosis by causing telomere dysfunction[J]. Oxid Med Cell Longev, 2023, 2023: 6897268.
    [17]
    WERNER C, HANHOUN M, WIDMANN T, et al. Effects of physical exercise on myocardial telomere-regulating proteins, survival pathways, and apoptosis[J]. J Am Coll Cardiol, 2008, 52(6): 470-482. doi: 10.1016/j.jacc.2008.04.034
    [18]
    GU J L, WANG S D, GUO H, et al. Inhibition of p53 prevents diabetic cardiomyopathy by preventing early-stage apoptosis and cell senescence, reduced glycolysis, and impaired angiogenesis[J]. Cell Death Dis, 2018, 9(2): 82. doi: 10.1038/s41419-017-0093-5
    [19]
    NAKAMURA H, MATOBA S, IWAI-KANAI E, et al. p53 promotes cardiac dysfunction in diabetic mellitus caused by excessive mitochondrial respiration-mediated reactive oxygen species generation and lipid accumulation[J]. Circ Heart Fail, 2012, 5(1): 106-115. doi: 10.1161/CIRCHEARTFAILURE.111.961565
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