Expression of circRNA ANKS1B and upstream transcription factor 1 in breast cancer tissues and its clinical significance

LIN Xiaolong; HUANG Xinyu; ZHOU Yizhi

doi:10.7619/jcmp.20222989

Journal of Clinical Medicine in Practice > 2023 > 27(5): 55-60. > DOI: 10.7619/jcmp.20222989

LIN Xiaolong, HUANG Xinyu, ZHOU Yizhi. Expression of circRNA ANKS1B and upstream transcription factor 1 in breast cancer tissues and its clinical significance[J]. Journal of Clinical Medicine in Practice, 2023, 27(5): 55-60. DOI: 10.7619/jcmp.20222989

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Expression of circRNA ANKS1B and upstream transcription factor 1 in breast cancer tissues and its clinical significance

1.
Department of Thyroid Gland and Mammary Gland Surgery, Qionghai City People′s Hospital of Hainan Province, Qionghai, Hainan, 571400
2.
Department of Pathology, Qionghai City People′s Hospital of Hainan Province, Qionghai, Hainan, 571400

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Received Date: September 23, 2022
Revised Date: November 09, 2022
Available Online: April 06, 2023

Graphical Abstract

Abstract

Abstract

Objective
To investigate the expression and clinical significance of CircRNA ANKS1B (CircANKS1B) and upstream transcription factor 1 (USF1) in breast cancer tissues.
Methods
A total of 90 patients with breast cancer were selected as subjects. USF1 protein expression in tissues was detected by immunohistochemistry. The expression of CircANKS1B and USF1 mRNA in tissues was detected by real-time quantitative polymerase chain reaction. The correlation between CircANKS1B and USF1 mRNA in cancer tissues was analyzed. The relationships of CircANKS1B and USF1 mRNA expression with clinicopathologic features of breast cancer were analyzed. The relationships of CircANKS1B and USF1 mRNA expression with prognosis of breast cancer were analyzed. Univariate and multivariate COX proportional risk models were used to analyze the independent influencing factors of the prognosis of breast cancer.
Results
The brownish-yellow expression of USF1 in breast cancer tissues was mainly located in the nucleus. The positive rate of USF1 protein expression in breast cancer tissues was 86.67% (78/90), which was significantly higher than 15.56% (14/90) in adjacent tissue (P < 0.001). The relative expression levels of CircANKS1B and USF1 mRNA in the breast cancer tissues were (4.12±0.68) and (6.35±1.21), respectively, which were significantly higher than (1.23± 0.46) and (1.57±0.59) in the adjacent tissue (P < 0.001). There was a significant positive correlation between CircANKS1B and USF1 mRNA expression in breast cancer tissues (r=0.604, P < 0.001). The expression of CircANKS1B and USF1 mRNA showed statistically significant differences in different tumor stages, lymph node metastasis and triple-negative breast cancer (P < 0.05). The median survival time of patients with high CircANKS1B expression was 52.62 months (95%CI, 49.44 to 56.35), which was significantly shorter than 56.78 months (95%CI: 52.46 to 57.05) in patients with low CircANKS1B expression (P=0.045). The median survival time of patients with low expression of USF1 mRNA was 52.53 months (95%CI, 48.83 to 56.23), which was significantly shorter than 56.75 months (95%CI, 54.07 to 59.43) of patients with low USF1 mRNA expression (P=0.014). CircANKS1B high expression, USF1 mRNA high expression, tumor stage Ⅲ and lymph node metastasis are independent influencing factors of the prognosis of breast cancer.
Conclusion
The expressions of CircANKS1B and USF1 are increased in breast cancer tissues, and correlated with TNM stage, lymph node metastasis, and are new breast cancer prognostic tumor markers.
- breast cancer,
- circRNA ANKS1B,
- upstream transcription factor 1,
- biomarker

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References (21)

References

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Expression of circRNA ANKS1B and upstream transcription factor 1 in breast cancer tissues and its clinical significance