Value of color Doppler ultrasound, ultrasound-guided fine needle aspiration cytology combined with <i>BRAF<sup>V600E</sup></i> gene detection in diagnosis of microthyroid nodules

QI Tiantian; LU Feng; HAN Xingxing; ZHOU Tianyan; WEI Jie; YAN Xinlin; ZHANG Qizhen; CAO Qi

doi:10.7619/jcmp.20222932

Journal of Clinical Medicine in Practice > 2023 > 27(5): 26-30. > DOI: 10.7619/jcmp.20222932

QI Tiantian, LU Feng, HAN Xingxing, ZHOU Tianyan, WEI Jie, YAN Xinlin, ZHANG Qizhen, CAO Qi. Value of color Doppler ultrasound, ultrasound-guided fine needle aspiration cytology combined with BRAF^V600E gene detection in diagnosis of microthyroid nodules[J]. Journal of Clinical Medicine in Practice, 2023, 27(5): 26-30. DOI: 10.7619/jcmp.20222932

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Value of color Doppler ultrasound, ultrasound-guided fine needle aspiration cytology combined with BRAF^V600E gene detection in diagnosis of microthyroid nodules

1.
Department of Endocrinology, Kunshan Integrated TCM and Western Medicine Hospital, Kunshan, Jiangsu, 215332
2.
Department of General Surgery, Kunshan Integrated TCM and Western Medicine Hospital, Kunshan, Jiangsu, 215332
3.
Community Health Service Center of Huaqiao Town in Kunshan City of Jiangsu Province, Kunshan, Jiangsu, 215332

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Received Date: September 18, 2022
Revised Date: January 06, 2023
Available Online: April 06, 2023

Graphical Abstract

Abstract

Abstract

Objective
To investigate the diagnostic value of color Doppler ultrasound, ultrasound-guided fine needle aspiration cytology (US-FNAC) combined with BRAF^V600E gene detection in small thyroid nodules.
Methods
A total of 103 patients with moderate or high risk small thyroid nodules were selected as study subjects. The Thyroid Imaging Reporting and Data System (TI-RADS) grading, preoperative color doppler ultrasound US-FNAC and silk/threonine specific kinase mutant gene V600E (BRAF^V600E) detection were performed. The sensitivity, specificity and accuracy of each examination alone and their combination were compared according to the pathological results, and the diagnostic performance was analyzed.
Results
The ultrasound examination showed that there were statistically significant differences in aspect ratio, structure, echo, echoes, calcification and marginal growth of nodules of different nature (P < 0.05). Of the 103 subjects, 63 cases (98 nodules with 67 malignant ones) were diagnosed as grade V or VI by US-FNAC examination, accounting for 61.2%. There were 26 patients with grade Ⅲ and Ⅳ (32 nodules with 14 malignant ones), accounting for 25.2%. There were 12 cases of grade Ⅱ (12 nodules in total without malignancy), accounting for 11.7%; there were 2 cases of grade Ⅰ (2 nodules with one malignant nodule), accounting for 1.9%. BRAF^V600E gene examination showed that 72 cases (111 nodules, 76 of which were malignant) was detected, accounting for 69.9%. There were 31 patients without mutation (33 nodules in total, 6 of which were malignant), accounting for 30.1%. The sensitivity, specificity and accuracy of ultrasound examination, US-FNAC examination and BRAF^V600E gene test were 75.3%, 62.5%, 73.0%, respectively, 79.5%, 68.8%, 77.5%, respectively and 93.2%, 68.8%, 88.8%, respectively. The sensitivity, specificity and accuracy of their combined diagnosis were the highest, which were 97.3%, 75.0% and 93.3%, respectively. The sensitivity, specificity and accuracy of their combined diagnosis were higher than those of single detection (P < 0.05).
Conclusion
Combined detection can improve the detection rate of thyroid papillary microcarcinoma, which is conducive to accurate preoperative evaluation of small nodules.

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References (21)

References

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Value of color Doppler ultrasound, ultrasound-guided fine needle aspiration cytology combined with BRAFV600E gene detection in diagnosis of microthyroid nodules

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Value of color Doppler ultrasound, ultrasound-guided fine needle aspiration cytology combined with BRAF^V600E gene detection in diagnosis of microthyroid nodules