Expression levels of microRNA-485-3p and Xklp2 target protein in breast cancer tissues and their clinical significance

LIU Peifa; HOU Wenyu; JIAN Yanbing

doi:10.7619/jcmp.20212629

Journal of Clinical Medicine in Practice > 2021 > 25(24): 29-34. > DOI: 10.7619/jcmp.20212629

LIU Peifa, HOU Wenyu, JIAN Yanbing. Expression levels of microRNA-485-3p and Xklp2 target protein in breast cancer tissues and their clinical significance[J]. Journal of Clinical Medicine in Practice, 2021, 25(24): 29-34. DOI: 10.7619/jcmp.20212629

Citation:

PDF (1603 KB)

Expression levels of microRNA-485-3p and Xklp2 target protein in breast cancer tissues and their clinical significance

the First Medical Center of General Hospital of the Chinese People's Liberation Army, Beijing, 100853

More Information

Received Date: June 24, 2021
Available Online: January 04, 2022
Published Date: December 27, 2021

Graphical Abstract

Abstract

Abstract

Objective To investigate the relationship between the expression levels of microRNA-485-3p (miR-485-3p) and Xklp2 target protein (TPX2) in breast cancer tissues and prognosis of patients.
Methods Immunohistochemistry was used to detect TPX2 protein expression, real-time fluorescent quantitative polymerase chain reaction was used to detect the expression of miR-485-3p and TPX2 mRNA. Pearson test was used to analyze the relationship between miR-485-3p expression and TPX2 mRNA expression in breast cancer tissues. Followed up for 5 years, the Kaplan-Meier method was used to analyze the relationships between the expressions of miR-485-3p, TPX2 proteins in breast cancer tissue and the overall survival rate of the patient, and COX regression analysis was used to analyze the risk factors affecting the overall survival of breast cancer patients.
Results The positive rate of TPX2 protein and the level of TPX2 mRNA expression in breast cancer tissues were significantly higher than those in normal tissues adjacent to cancer, the expression level of miR-485-3p was significantly lower than that of normal tissues adjacent to cancer (P < 0.05). The expression of miR-485-3p and TPX2 proteins in breast cancer tissues were related to TNM stage, lymph node metastasis and Ki-67 expression (P < 0.05). The expression of miR-485-3p in breast cancer tissue was negatively correlated with the expression of TPX2 mRNA (P < 0.05). The 5-year overall survival rate of patients with high miR-485-3p expression was 77.78%, which was significantly higher than 52.94% of patients with low miR-485-3p expression (P < 0.05); the 5-year overall survival rate of TPX2 negative patients was 84.00%, which was significantly higher than 60.00% of positive patients (P < 0.05). The lymph node metastasis, low expression of miR-485-3p, and positive TPX2 protein were independent risk factors affecting the overall survival of breast cancer patients (P < 0.05).
Conclusion In breast cancer tissues, miR-485-3p is lowly expressed and TPX2 is highly expressed. They are negatively correlated, and both can be used as prognostic indicators for judging breast cancer patients.
- breast cancer,
- microRNA-485-3p,
- Xklp2 target protein,
- prognosis

FullText(HTML)

References (16)

References

[1]	VERRET B, SOURISSEAU T, STEFANOVSKA B, et al. The influence of cancer molecular subtypes and treatment on the mutation spectrum in metastatic breast cancers[J]. Cancer Res, 2020, 80(15): 3062-3069. doi: 10.1158/0008-5472.CAN-19-3260
[2]	马跃, 高英静, 何浪. 乳腺癌差异表达miRNA在预后中的意义[J]. 医学研究杂志, 2018, 47(1): 39-44. https://www.cnki.com.cn/Article/CJFDTOTAL-YXYZ201801013.htm
[3]	艾尼·沙塔尔, 闫焕英, 丁伟, 等. 长链非编码RNA MALAT1靶向miR-485-3p调控乳腺癌细胞对紫杉醇的耐药性[J]. 南方医科大学学报, 2020, 40(5): 698-702. https://www.cnki.com.cn/Article/CJFDTOTAL-DYJD202005014.htm
[4]	JIANG Y Q, LIU Y, TAN X L, et al. TPX2 as a novel prognostic indicator and promising therapeutic target in triple-negative breast cancer[J]. Clin Breast Cancer, 2019, 19(6): 450-455. doi: 10.1016/j.clbc.2019.05.012
[5]	TAHERDANGKOO K, KAZEMINEZHAD S R, HAJJARI M R, et al. miR-485-3p suppresses colorectal cancer via targeting TPX2[J]. Bratislava Med J, 2020, 121(4): 302-307. doi: 10.4149/BLL_2020_048
[6]	中国抗癌协会乳腺癌专业委员会. 中国抗癌协会乳腺癌诊治指南与规范(2017年版)[J]. 中国癌症杂志, 2017, 27(9): 695-759. https://www.cnki.com.cn/Article/CJFDTOTAL-ZGAZ201709004.htm
[7]	WANG Y W, ZHAO S, YUAN X Y, et al. miR-4732-5p promotes breast cancer progression by targeting TSPAN13[J]. J Cell Mol Med, 2019, 23(4): 2549-2557. doi: 10.1111/jcmm.14145
[8]	许发功, 杨立. 乳腺癌组织中miR-144-3p和SGK3的表达水平及临床意义[J]. 中国临床研究, 2019, 32(2): 173-178. https://www.cnki.com.cn/Article/CJFDTOTAL-ZGCK201902008.htm
[9]	LAI J J, XIN J, FU C D, et al. CircHIPK3 promotes proliferation and metastasis and inhibits apoptosis of renal cancer cells by inhibiting MiR-485-3p[J]. Cancer Cell Int, 2020, 20: 248. doi: 10.1186/s12935-020-01319-3
[10]	WANG M, CAI W R, MENG R, et al. miR-485-5p suppresses breast cancer progression and chemosensitivity by targeting survivin[J]. Biochem Biophys Res Commun, 2018, 501(1): 48-54. doi: 10.1016/j.bbrc.2018.04.129
[11]	WANG X, ZHOU X, ZENG F, et al. miR-485-5p inhibits the progression of breast cancer cells by negatively regulating MUC1[J]. Breast Cancer, 2020, 27(4): 765-775. doi: 10.1007/s12282-020-01075-2
[12]	王庆亮, 赵艳峰, 蒋燕, 等. TPX2与肿瘤关系的研究进展[J]. 实用肿瘤学杂志, 2021, 35(1): 83-86. https://www.cnki.com.cn/Article/CJFDTOTAL-SYZL202101022.htm
[13]	JIANG T C, SUI D M, YOU D, et al. RETRACTED ARTICLE: MiR-29a-5p inhibits proliferation and invasion and induces apoptosis in endometrial carcinoma via targeting TPX2[J]. Cell Cycle, 2018, 17(10): 1268-1278. doi: 10.1080/15384101.2018.1475829
[14]	ZOU J, HUANG R Y, JIANG F N, et al. Overexpression of TPX2 is associated with progression and prognosis of prostate cancer[J]. Oncol Lett, 2018, 16(3): 2823-2832. http://www.ingentaconnect.com/content/sp/ol/2018/00000016/00000003/art00008
[15]	ZHOU F, WANG M, AIBAIDULA M, et al. TPX2 promotes metastasis and serves as a marker of poor prognosis in non-small cell lung cancer[J]. Med Sci Monit, 2020, 26: e925147. https://pubmed.ncbi.nlm.nih.gov/32748897/#:~:text=TPX2%20Promotes%20Metastasis%20and%20Serves%20as%20a%20Marker,mechanisms%20would%20aid%20in%20initiating%20effective%20clinical%20treatment.
[16]	TAN G Z, LI M, TAN X, et al. MiR-491 suppresses migration and invasion via directly targeting TPX2 in breast cancer[J]. Eur Rev Med Pharmacol Sci, 2019, 23(22): 9996-10004. http://www.ncbi.nlm.nih.gov/pubmed/31799669

Cited By

Cited by

Periodical cited type(2)

1.	刘红玲，张保萍，吕素英. 宫颈癌组织中TPX2的表达水平及其与放疗敏感性的相关性. 实用癌症杂志. 2024(06): 921-924 .
2.	嵇晓辉，姜丽娜，王娜，刘薇，杨金花. miRNA-455-3p在乳腺癌组织中的表达及与紫杉醇耐药的关系. 癌症进展. 2023(02): 146-149 .

Other cited types(0)

Get Citation

PDF

XML

Article views (262) PDF downloads (14) Cited by(2)

Turn off MathJax

Article Contents

Abstract

References

Expression levels of microRNA-485-3p and Xklp2 target protein in breast cancer tissues and their clinical significance

Abstract

References

Cited by

Periodical cited type(2)

Other cited types(0)

Catalog

Export File

Citation

Format

Content