Expressions of miR-125a-5p, signal transducer and activator of transcription 3 in gastric cancer and their biological functions

TANG Xiaolong; CHEN Xufeng; ZHENG Yang; YANG Shenglan

doi:10.7619/jcmp.20212363

Journal of Clinical Medicine in Practice > 2021 > 25(20): 61-67. > DOI: 10.7619/jcmp.20212363

TANG Xiaolong, CHEN Xufeng, ZHENG Yang, YANG Shenglan. Expressions of miR-125a-5p, signal transducer and activator of transcription 3 in gastric cancer and their biological functions[J]. Journal of Clinical Medicine in Practice, 2021, 25(20): 61-67. DOI: 10.7619/jcmp.20212363

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Expressions of miR-125a-5p, signal transducer and activator of transcription 3 in gastric cancer and their biological functions

Department of Gastroenterology, the 904^th Hospital of Joint Logistics and Support Force of Chinese People's Liberation Army, Wuxi, Jiangsu, 214000

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Received Date: June 07, 2021
Available Online: November 15, 2021
Published Date: October 27, 2021

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Abstract

Abstract

Objective To verify the expression of miR-125a-5p, signal transducer and activator of transcription 3 (STAT3) in gastric cancer and their biological functions.
Methods Gastric cancer tissues were isolated from 30 patients with gastric cancer, and real-time fluorescent quantitative PCR (qRT-PCR) and Western blot (WB) were used to detect expression of miR-125a-5p and STAT3 in gastric cancer tissue and non-cancerous gastric tissue. After cell transfection, Cell Counting Kit-8 (CCK-8) was used to detect cell viability, and flow cytometry was used to detect cell cycle and apoptosis. Transwell migration test was used to detect migrating cells. The target relationship between miR-125a-5p and STAT3 was verified by dual luciferase reporter gene detection. The tumor inhibitory effect of miR-125a-5p was verified by tumor formation experiment in nude mice in vivo.
Results The expression of miR-125a-5p in gastric cancer tissue was significantly lower than that in non-cancerous gastric tissue (P < 0.05). In gastric cancer cell lines, the expression of miR-125a-5p was also significantly lower than that in the normal epithelial cells (P < 0.05). In addition, authors transfected gastric cancer cells with miR-125a-5p mimics, and the results showed that the over-expression of miR-125a-5p was able to inhibit the proliferation, migration and invasion of gastric cancer cells by targeting STAT3. Tumor formation experiment in nude mice verified that over-expression of miR-125a-5p was able to inhibit tumor proliferation and promote tumor cell apoptosis.
Conclusion MiR-125a-5p can inhibit tumors by inhibiting the expression of STAT3, and these findings will provide reliable theoretical bases for clinical targeted therapy and early selection of biomarkers.
- gastric cancer,
- miR-125a-5p,
- signal transducer and activator of transcription 3,
- tumor progression

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