Effect of luteolin on the proliferation, apoptosis and Hippo/Yes-related protein pathway of lung epithelial cells induced by bleomycin

JIANG Xiuchun; WANG Dejun; YU He

doi:10.7619/jcmp.20211773

Journal of Clinical Medicine in Practice > 2021 > 25(21): 83-87. > DOI: 10.7619/jcmp.20211773

JIANG Xiuchun, WANG Dejun, YU He. Effect of luteolin on the proliferation, apoptosis and Hippo/Yes-related protein pathway of lung epithelial cells induced by bleomycin[J]. Journal of Clinical Medicine in Practice, 2021, 25(21): 83-87. DOI: 10.7619/jcmp.20211773

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Effect of luteolin on the proliferation, apoptosis and Hippo/Yes-related protein pathway of lung epithelial cells induced by bleomycin

Department of Respiratory Medicine, Panjin Liaoyou Baoshihua Hospital in Liaoning Province, Panjin, Liaoning, 124010

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Received Date: April 26, 2021
Available Online: December 02, 2021
Published Date: November 14, 2021

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Abstract

Abstract

Objective To explore the effect of luteolin(Lut)on the proliferation, apoptosis and the Hippo/Yes-related protein (YAP) pathway of human lung epithelial cell (BEAS-2B cells) induced by bleomycin(BLM).
Methods BEAS-2B cells were cultured in vitro, and induced with 4, 8, 12 and 24 μg/mL BLM for 12, 24 and 48 h, respectively, to determine the BLM induction concentration and time. The BEAS-2B cells were divided into control group, BLM group, and Lut low, medium and high concentration groups. After 24 hours of cell culture in each group, the morphological changes of the cells were observed with an inverted microscope; the cell proliferation ability was detected by the CCK-8 method; the rate of cell apoptosis was detected by flow cytometry; Western blot was used to detect Hippo/YAP pathway related proteins-mammalian Sterile20-like 1(MST1), the large tumour suppressor 1 (LAST1) and YAP phosphorylation levels.
Results The half maximal inhibitory concentration (IC50) of BEAS-2B cells after 24 hours of BLM induction was about 8 μg/mL, so 8 μg/mL BLM was selected to induce cells for 24 hours. Compared with the control group, the BLM group had smaller cells, irregular edges, increased intercellular space, the cell proliferation ability, MST1, LAST1 and YAP phosphorylation levels significantly decreased, and apoptosis rate significantly increased (P < 0.05); compared with the BLM group, the cell morphology of the Lut low, medium, and high concentration groups tended to be normal, and the cell proliferation ability, MST1, LAST1 and YAP protein phosphorylation levels increased successively (P < 0.05), and the rate of apoptosis decreased successively (P < 0.05).
Conclusion Lut may activate the Hippo/YAP signaling pathway in BEAS-2B cells, increase the proliferation of BEAS-2B cells induced by BLM, and inhibit cell apoptosis.
- luteolin,
- bleomycin,
- lung epithelial cells,
- Hippo/Yes-associated protein pathway,
- proliferation,
- apoptosis

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References (23)

References

[1]	杨航, 张红. 肺泡Ⅱ型上皮细胞与肺部疾病的研究进展[J]. 安徽医药, 2021, 25(2): 217-221. doi: 10.3969/j.issn.1009-6469.2021.02.002
[2]	李莉, 王永艳, 成梦群, 等. 龙胆苦苷对博来霉素诱导肺泡上皮细胞凋亡的抑制作用[J]. 昆明医科大学学报, 2019, 40(6): 11-15. doi: 10.3969/j.issn.1003-4706.2019.06.003
[3]	高琳, 费静, 刘毅, 等. 木犀草素对下咽癌细胞FaDu抑制的作用机制研究[J]. 中国临床药理学杂志, 2020, 36(21): 3497-3499, 3518. https://www.cnki.com.cn/Article/CJFDTOTAL-GLYZ202021025.htm
[4]	董欣敏, 郑媞, 张子英, 等. 木犀草素通过逆转OPCML基因甲基化抑制乳腺癌MDA-MB-231细胞增殖[J]. 南方医科大学学报, 2020, 40(4): 550-555. https://www.cnki.com.cn/Article/CJFDTOTAL-DYJD202004017.htm
[5]	胡艺还, 高静, 杨宁刚, 等. 木犀草素通过抑制Akt/IKK信号通路抑制膀胱癌5637细胞迁移、侵袭和上皮间质转化[J]. 中国药理学通报, 2020, 36(8): 1083-1088. doi: 10.3969/j.issn.1001-1978.2020.08.010
[6]	齐海霞, 柴艳芬. Hippo信号通路相关分子与肿瘤发生的研究进展[J]. 医学综述, 2020, 26(4): 683-689. doi: 10.3969/j.issn.1006-2084.2020.04.012
[7]	李朗, 董亮, 高飞, 等. Hippo信号通路调控间充质干细胞对ARDS肺损伤修复效应的实验研究[J]. 中华危重病急救医学, 2019(3): 281-287. doi: 10.3760/cma.j.issn.2095-4352.2019.03.005
[8]	ZHAO S, XU K, JIANG R, et al. Evodiamine inhibits proliferation and promotes apoptosis of hepatocellular carcinoma cells via the Hippo-Yes-Associated Protein signaling pathway[J]. Life Sci, 2020, 251: 117424. doi: 10.1016/j.lfs.2020.117424
[9]	缪存静, 陈俊杰, 历星, 等. 木犀草素逆转由TGF-β1诱导的人肺癌细胞上皮-间充质转化的实验研究[J]. 中国病理生理杂志, 2019, 35(7): 1163-1168. doi: 10.3969/j.issn.1000-4718.2019.07.002
[10]	刘燕, 周静珠, 周广辉, 等. C/EBPβ与肺上皮细胞间充质转化及肺纤维化的研究进展[J]. 临床肺科杂志, 2019, 24(8): 1540-1543. doi: 10.3969/j.issn.1009-6663.2019.08.045
[11]	亓水芹, 冯向功, 王慧琴. miR-194靶向FoxA1基因对LPS诱导的肺泡上皮细胞损伤影响[J]. 免疫学杂志, 2020, 36(3): 193-200. https://www.cnki.com.cn/Article/CJFDTOTAL-MYXZ202003002.htm
[12]	刘思伽, 全紫瑶, 胡必成, 等. Hippo-YAP通路与肿瘤相关性研究进展[J]. 医学研究杂志, 2019, 48(7): 12-15, 27. https://www.cnki.com.cn/Article/CJFDTOTAL-YXYZ201907006.htm
[13]	王清岑, 殷新, 周罗慧, 等. 木犀草素心血管保护作用研究进展[J]. 中国现代医学杂志, 2020, 30(11): 57-60. doi: 10.3969/j.issn.1005-8982.2020.11.012
[14]	WU H T, LIN J, LIU Y E, et al. Luteolin suppresses androgen receptor-positive triple-negative breast cancer cell proliferation and metastasis by epigenetic regulation of MMP9 expression via the AKT/mTOR signaling pathway[J]. Phytomedicine, 2021, 81: 153437. doi: 10.1016/j.phymed.2020.153437
[15]	任开明, 周兆丽, 石文君. 木犀草素对人非小细胞肺癌A549细胞增殖、凋亡、侵袭及迁移能力的影响[J]. 解剖科学进展, 2019, 25(4): 361-363, 367. https://www.cnki.com.cn/Article/CJFDTOTAL-JPKX201904004.htm
[16]	王丽芳, 赵方毓, 唐鲜娥, 等. 木犀草素通过线粒体凋亡途径诱导SW480结肠癌细胞的凋亡作用研究[J]. 中国临床药理学杂志, 2019, 35(17): 1862-1864, 1875. https://www.cnki.com.cn/Article/CJFDTOTAL-GLYZ201917008.htm
[17]	刘宏飞, 候亚鹏, 金睿, 等. 木犀草素通过调节上皮钠通道影响肺上皮离子转运[J]. 中药新药与临床药理, 2020, 31(4): 402-408. https://www.cnki.com.cn/Article/CJFDTOTAL-ZYXY202004007.htm
[18]	孙力超, 陈旭, 姚尧, 等. 木犀草素对脓毒症小鼠急性肺损伤的保护作用[J]. 中华急诊医学杂志, 2019, 28(6): 717-723. doi: 10.3760/cma.j.issn.1671-0282.2019.06.012
[19]	尚璐, 张骁, 陈岩, 等. Hippo/YAP信号通路调控蛋白ACAN作为新型肝细胞肝癌血清肿瘤标志物的研究[J]. 检验医学, 2019, 34(1): 60-66. https://www.cnki.com.cn/Article/CJFDTOTAL-SHYY201901015.htm
[20]	陈庆, 殷焦. Hippo通路在感染及肿瘤免疫中的相关作用研究进展[J]. 解放军医学杂志, 2019, 44(9): 791-796. https://www.cnki.com.cn/Article/CJFDTOTAL-JFJY201909015.htm
[21]	KIM S H, JIN H, MENG R Y, et al. Activating hippo pathway via Rassf1 by ursolic acid suppresses the tumorigenesis of gastric cancer[J]. Int J Mol Sci, 2019, 20(19): 4709. doi: 10.3390/ijms20194709
[22]	俞晓军. 尼可地尔对肺动脉平滑肌细胞增殖、迁移能力和hippo/YAP信号通路的影响[J]. 中国药房, 2020, 21(22): 2736-2740. doi: 10.6039/j.issn.1001-0408.2020.22.09
[23]	ZUO W Y, LIU N, ZENG Y H, et al. Luteolin ameliorates experimental pulmonary arterial hypertension via suppressing hippo-YAP/PI3K/AKT signaling pathway[J]. Front Pharmacol, 2021, 12: 663551. doi: 10.3389/fphar.2021.663551