Experimental study on apoptosis of gastric cancer cell line SGC-7901 induced by coix seed oil

SUN Guozhuang; CAO Youjun; MAO Haiyan; LUO Heng; HE Zidu; KONG Guimei

doi:10.7619/jcmp.201908001

Journal of Clinical Medicine in Practice > 2019 > 23(8): 1-6. > DOI: 10.7619/jcmp.201908001

SUN Guozhuang, CAO Youjun, MAO Haiyan, LUO Heng, HE Zidu, KONG Guimei. Experimental study on apoptosis of gastric cancer cell line SGC-7901 induced by coix seed oil[J]. Journal of Clinical Medicine in Practice, 2019, 23(8): 1-6. DOI: 10.7619/jcmp.201908001

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Experimental study on apoptosis of gastric cancer cell line SGC-7901 induced by coix seed oil

1.
Institute of Translational Medicine, Medical College of Yangzhou University, Yangzhou, Jiangsu, 225001
2.
Department of Clinical Laboratory, Xuyi People′s Hospital, Huai′an, Jiangsu, 211700
3.
Department of Chemotherapy, Nanjing BenQ Hospital, Nanjing, Jiangsu, 210000

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Received Date: January 10, 2019
Accepted Date: February 27, 2019
Available Online: December 03, 2020
Published Date: April 27, 2019

Graphical Abstract

Abstract

Abstract

Objective To investigate the possible mechanism of coix seed in inducing apoptosis of gastric cancer cells (SGC-7901).
Methods Different concentrations of coix seed oil (2, 4, 8 mg/mL) were applied to SGC-7901 cells. MTT assay was used to detect the effect of drugs on cell proliferation, and flow cytometry for drug-induced cell apoptosis, scratch test for cell migration inhibited by coix seed oil, Transwell chamber for drug-inhibited cell invasion, and Western blot for expression of related proteins PRMT5, PI3K and AKT.
Results The results of MTT showed that 2, 4 mg/mL of coix seed oil could significantly inhibit the proliferation of SGC-7901 cells, and the inhibition rate of 2 mg/mL was (30.02±1.56)%, which showed significant difference compared to the control group (P < 0.01). The results of flow cytometry showed that the apoptosis rates of coix seed oil cells at concentrations of 2 and 4 mg/mL were (16.25±2.54)%, (12.60±1.12)%, respectively, and which showed a significant difference compared with (2.0±1.22)% in the control group (P < 0.01). The results of cell scratch test showed that the migration of SGC-7901 cells treated with 2, 4 mg/mL of coix seed oil had significant difference when compared to control group (P < 0.01). The results of invasion experiments showed that 2, 4 mg/mL of coix seed oil could significantly inhibit cell invasion, and the number of cell invasion was(134.00±2.86), (167.00±0.99), respectively, which showed significant difference compared to(268.00±2.05)in the control group (P < 0.01). The migration number in 8 mg/mL coix seed oil group was (167±0.99), a significant difference was observed when compared to the control group(P < 0.05). Western blot analysis showed that different concentrations of coix seed oil could significantly down-regulate the expression of PRMT5, PI3K and AKT in SGC-7901 cells.
Conclusion Coix seed oil can significantly inhibit the proliferation, migration and invasion of gastric cancer SGC-7901 cells, its possible mechanism is to down-regulate the signal pathway of PRMT5-PI3K/AKT to inhibit the activation of various anti-apoptotic molecules, induce apoptosis and suppress invasion and metastasis of gastric cancer SGC-7901 cells by down-regulating the PRMT5-PI3K/AKT signaling pathway.
- coix seed oil,
- gastric cancer,
- protein arginine methyltransferase 5,
- migration

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References (23)

References

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