血清人类β防御素2、可溶性生长刺激表达基因2蛋白表达水平对儿童难治性支原体肺炎的诊断价值

Diagnostic value of serum human β-defensin 2 and soluble growth stimulating gene 2 expression levels in pediatric refractory Mycoplasma pneumonia

  • 摘要:
    目的 探讨血清人类β防御素2(HBD2)和可溶性生长刺激表达基因2蛋白(sST2)水平对儿童难治性支原体肺炎的诊断价值。
    方法 选取145例支原体肺炎患儿为研究对象,根据是否患有难治性支原体肺炎分为难治性肺炎组(n=53)和普通肺炎组(n=92)。比较2组一般资料。采用酶联免疫吸附测定法检测2组血清HBD2、sST2水平。采用多因素Logistic回归分析法筛选难治性支原体肺炎发生的影响因素。采用受试者工作特征(ROC)曲线分析血清HBD2、sST2水平对难治性支原体肺炎的诊断价值。
    结果 难治性肺炎组的肺实变占比、胸腔积液占比高于普通肺炎组,发热时间长于普通肺炎组,差异有统计学意义(P < 0.05)。难治性肺炎组的血清HBD2、sST2水平高于普通肺炎组,差异有统计学意义(P < 0.05)。多因素Logistic回归分析表明,肺实变、胸腔积液、高HBD2血清水平和高sST2血清水平为儿童难治性支原体肺炎的危险因素(P < 0.05)。血清HBD2水平诊断儿童难治性支原体肺炎的曲线下面积、Youden指数分别为0.817、0.557, 血清sST2水平诊断的曲线下面积、Youden指数分别为0.841、0.607。二者联合诊断儿童难治性支原体肺炎的ROC曲线下面积为0.916, Youden指数为0.721。
    结论 血清HBD2、sST2水平联合诊断儿童难治性支原体肺炎的价值较高。

     

    Abstract:
    Objective To investigate the diagnostic value of serum human β-defensin 2 (HBD2) and soluble growth stimulating gene 2 (sST2) levels in pediatric refractory Mycoplasma pneumonia.
    Methods A total of 145 children diagnosed with Mycoplasma pneumonia were recruited, and divided into refractory pneumonia group (n=53) and common pneumonia group (n=92) based on whether they had refractory or common pneumonia. General data were compared between the two groups. Serum HBD2 and sST2 levels were measured using enzyme-linked immunosorbent assay (ELISA). Multivariate logistic regression analysis was used to identify influencing factors for the occurrence of refractory Mycoplasma pneumonia. Receiver operating characteristic (ROC) curve analysis was performed to evaluate the diagnostic value of serum HBD2 and sST2 levels in refractory Mycoplasma pneumonia.
    Results The proportion of lung consolidation and pleural effusion in refractory pneumonia group was significantly higher, and the fever time was significantly longer than that in common pneumonia group (P < 0.05). Serum HBD2 and sST2 levels in the refractory pneumonia group were significantly higher than those in the common pneumonia group (P < 0.05). Multivariate Logistic regression analysis indicated that lung consolidation, pleural effusion, high serum HBD2 level and high serum sST2 level were risk factors for pediatric refractory Mycoplasma pneumonia (P < 0.05). The area under the curve and Youden's index for diagnosing pediatric refractory Mycoplasma pneumonia were 0.817 and 0.557 for serum HBD2 level, and 0.841 and 0.607 for serum sST2 level, respectively. Combined diagnosis using both markers resulted in an area under the curve of 0.916 and a Youden′s index of 0.721.
    Conclusion The combined detection of serum HBD2 and sST2 levels holds significant diagnostic value for pediatric refractory Mycoplasma pneumonia.

     

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