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甲状腺癌组织中微小RNA-495-3p、Holliday交叉识别蛋白表达水平及临床意义

Expression levels and clinical significance of microR-495-3p and Holliday junction recognition protein in thyroid cancer

    摘要
  • 摘要:
      目的  检测甲状腺癌组织中微小RNA-495-3p(miR-495-3p)、Holliday交叉识别蛋白(HJURP)的表达水平及临床意义。
      方法  选择行手术根治术切除并经病理检查确诊的甲状腺癌石蜡包埋标本67例及正常甲状腺组织石蜡包埋标本42例为研究对象，利用荧光定量聚合酶链反应(PCR)技术检测miR-495-3p水平，采用免疫组织化学法检测HJURP阳性表达情况，分析miR-495-3p、HJURP水平与甲状腺癌患者临床病理参数的关系。采用Kaplan-Meier生存曲线分析甲状腺癌组织中miR-495-3p、HJURP水平与患者预后的关系; 采用COX回归分析探讨影响甲状腺癌患者预后的不良因素。
      结果  HJURP在癌组织中的阳性表达率为62.69%, 在正常甲状腺组织中的阳性表达率为26.19%, 差异有统计学意义(χ2=13.765, P < 0.01); miR-495-3p在甲状腺癌组织和正常甲状腺组织中的相对表达量分别为(0.76±0.21)、(1.26±0.40), 差异有统计学意义(t=8.539, P < 0.001); miR-495-3p、HJURP水平与甲状腺患者临床分期、淋巴结转移有关(P < 0.05); 临床分期Ⅲ~Ⅳ、发生淋巴结转移的甲状腺癌患者miR-495-3p的水平更低, HJURP蛋白阳性表达更高; miR-495-3p高表达患者3年累积生存率高于miR-495-3p低表达组，差异有统计学意义(χ2=8.478, P=0.004); HJURP阳性患者3年累积生存率为57.14%, 低于HJURP阴性患者的92.00%, 差异有统计学意义差异有统计学意义(χ2=9.093, P=0.003)。多因素分析结果显示，临床分期、淋巴结转移、miR-495-3p、HJURP是影响甲状腺癌患者预后不良的独立危险因素(P < 0.05)。
      结论  甲状腺癌组织中miR-495-3p表达下调, HJURP表达上调，与肿瘤临床分期、淋巴结转移及预后有关，可能参与了肿瘤细胞的分化、迁移等过程。

     

    Abstract:
      Objective  To detect the expression levels of microRNA-495-3p (miR-495-3p) and Holliday junction recognition protein (HJURP) in thyroid cancer tissues and explore their clinical significance.
      Methods  A total of 67 paraffin-embedded specimens of thyroid cancer diagnosed by pathological examination and 42 paraffin-embedded specimens of normal thyroid tissue were selected as research objects. The miR-495-3p level was detected with fluorescence quantitative polymerase chain reaction (PCR) technology, the positive expression of HJURP was detected by immunohistochemistry, the relationships of miR-495-3p, HJURP levels with clinicopathological parameters in patients with thyroid cancer were analyzed. Kaplan-Meier survival curve was used to analyze the relationships of the levels of miR-495-3p and HJURP in thyroid cancer with the prognosis ofpatients; COX regression analysis was used to explore the adverse factors affecting the prognosis of thyroid cancer patients.
      Results  The positive expression rate of HJURP in cancer tissues was 62.69%, and was 26.19% in normal thyroid tissues (P < 0.01); the relative expression of miR-495-3p in thyroid cancer tissue and normal thyroid tissue was (0.76±0.21) and (1.26±0.40), respectively(t=8.539, P < 0.001); the levels of miR-495-3p and HJURP were related to the clinical stageand lymph node metastasis of thyroid patients (P < 0.05). The levels of miR-495-3p in patients with thyroid cancer in clinical stage of Ⅲ to Ⅳ and lymph nodemetastasis were lower, and their positive expression of HJURP protein were higher; the 3-year cumulative survival rates of patients with miR-495-3p high expression were significantly higher than in those with miR-495-3p low expression (χ2=8.478, P=0.004); the 3-year cumulative survival rate of patients with positive HJURP was significantly lower than that in those with negative HJURP (57.14% versus 92.00%, χ2=9.093, P=0.003); the results of multivariate analysis showed that clinical staging, lymph node metastasis, miR-495-3p, and HJURP were independent risk factors affecting the poor prognosis of patients with thyroid cancer (P < 0.05).
      Conclusion  In tissues of thyroid cancer, the expression of miR-495-3p is down-regulated, and the expression of HJURP is up-regulated, which are related to the clinical stage of the tumor, lymph node metastasis and prognosis, and may be involved in the differentiation and migration of tumor cells.

     

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