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微小RNA-126和血管内皮生长因子在增殖性糖尿病视网膜病变患者视网膜前膜中的表达及意义

Expressions and significance of microRNA-126 and vascular endothelial growth factor in epiretinal membrane of patients with proliferative diabetic retinopathy

    摘要
  • 摘要:
      目的  探讨微小RNA-126(miR-126)和血管内皮生长因子(VEGF)在增殖性糖尿病视网膜病变(PDR)患者视网膜前膜组织中的表达及临床意义。
      方法  选取眼科择期行玻璃体切割手术的PDR患者65例纳入研究组, 另选取同期行玻璃体切割手术的特发性黄斑裂孔患者62例纳入对照组。采用实时荧光定量聚合酶链反应(qRT-PCR)法检测2组患者视网膜前膜组织中miR-126、VEGF mRNA表达水平，采用Western blotting法检测VEGF蛋白表达水平，检测患者空腹血糖(FBG)、甘油三酯(TG)、糖化血红蛋白(HbA1c)水平，采用Pearson相关分析法分析miR-126与VEGF mRNA的相关性及两者与FGB、HbA1c、TG的相关性，并对PDR发生的影响因素进行Logistic多元回归分析。
      结果  研究组患者视网膜前膜组织中miR-126表达水平低于对照组, VEGF mRNA及其蛋白表达水平高于对照组，差异有统计学意义(P < 0.05); 研究组患者FBG、TG和HbA1c水平均高于对照组，差异有统计学意义(P < 0.05); miR-126与VEGF mRNA呈显著负相关(P < 0.05); miR-126与FGB、HbA1c、TG呈显著负相关(P < 0.05), 而VEGF mRNA与FGB、HbA1c、TG呈显著正相关(P < 0.05); Logistic多元回归分析显示, miR-126与VEGF mRNA表达水平均是患者发生PDR的独立影响因素。
      结论  PDR患者视网膜前膜组织中miR-126表达显著降低, VEGF表达显著升高, miR-126可能通过负性调控VEGF表达而参与PDR的发生发展。

     

    Abstract:
      Objective  To investigate expressions of microRNA-126(miR-126) and vascular endothelial growth factor (VEGF) in the epiretinal membrane tissues of patients with proliferative diabetic retinopathy (PDR) and their clinical significance.
      Methods  A total of 65 patients with PDR who underwent elective vitrectomy were selected as study group, and 62 patients with idiopathic macular hole who underwent vitrectomy at the same time were selected as control group. Quantitative Real-time Polymerase Chain Reaction(qRT-PCR) was used to detect the expression levels of miR-126 and VEGF mRNA in the epiretinal membrane tissues of the two groups; western blotting assay was used to detect the expression level of VEGF protein; fasting blood glucose (FBG), triglyceride (TG) and glycosylated hemoglobin (HbA1c) levels were detected; Pearson correlation analysis was used to analyze the correlation between miR-126 and VEGF mRNA and their correlations with FGB, HbA1c and TG were analyzed; Logistic multiple regression analysis was used to analyze the influencing factors for the occurrence of PDR.
      Results  The expression level of miR-126 in the epiretinal membrane of patients in the study group was significantly lower than that in the control group, VEGF mRNA and protein expression levels in the study group were significantly higher than those in the control group (P < 0.05). The levels of FGB, TG and HbA1c in the study group were significantly higher than those in the control group (P < 0.05). There were negative correlations of miR-126 with VEGF mRNA, FGB, HbA1c and TG (P < 0.05), and VEGF mRNA was positively correlated with FGB, HbA1c and TG (P < 0.05). Logistic multiple regression analysis showed that both miR-126 and VEGF mRNA were independent influencing factors for PDR in patients.
      Conclusion  The expression of miR-126 is decreased in the epiretinal membrane tissues of PDR patients, while the expression of VEGF is significantly increased. Therefore, miR-126 may be involved in the development and progression of PDR through negative regulation of VEGF expression.

     

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