Expression of interleukin-13 receptor α2 in cancer cells of colorectal cancer patients with liver metastasis and its relationship with myeloid-derived suppressor cells
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摘要: 目的 探讨白细胞介素-13受体α2(IL-13Rα2)在结直肠癌肝转移患者细胞中的表达及其与骨髓源性抑制细胞(MDSCs)的关系。 方法 回顾性分析2000—2013年复旦大学附属华山医院手术的68例结直肠癌肿瘤患者石蜡切片,使用常规免疫染色方案,切片观察IL-13Rα2表达。采用抗CD11B抗体和抗CD33抗体进行双重染色,采用荧光抗体、山羊抗兔免疫球蛋白(H+L)抗体和山羊抗鼠免疫球蛋白(H+L)抗体对CD33进行显示。3位病理专家进行双盲独立评估IL-13Rα2染色载玻片。 结果 结直肠癌肝转移患者IL-13Rα2阳性率最高(90.8%), 其次为脑转移(65.0%)、原发病灶(60.0%)、其他转移(53.0%), 差异有统计学意义(P<0.05)。在肝转移病例中, IL-13Rα2表达与MDSCs数量呈线性相关(r=0.740, P<0.05)。48例远处转移病例的平均生存期为(12.6±5.2)个月。 结论 IL-13Rα2水平升高提示结直肠癌远处转移患者的预后较差,推测IL-13Rα2通过调节结直肠癌细胞来诱导MDSCs增殖并促进肿瘤肝转移。
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关键词:
- 白细胞介素-13受体 /
- 骨髓源性抑制细胞 /
- 结直肠癌 /
- 肝转移 /
- 脑转移
Abstract: Objective To investigate the expression of interleukin-13 receptor α2(IL-13Rα2)in cells of colorectal cancer patients with liver metastasis and its relationship with myeloid-derived suppressor cells(MDSCs). Methods Paraffin sections of 68 patients with colorectal cancer surgery in Huashan Hospital Affiliated to Fudan University from 2000 to 2013 were retrospectively analyzed. The expression of IL-13Rα2 was observed after routine immunostaining. Anti-CD11B antibody and anti-CD33 antibody were used for double staining. Fluorescent antibody, goat anti-rabbit immunoglobulin(H+L)antibody and goat anti-mouse immunoglobulin(H+L)antibody were used to display CD33. Three pathologists independently evaluated IL-13Rα2 stained slides in a double-blind manner. Results The positive rate of IL-13Rα2 in colorectal cancer patients with liver metastasis was the highest(90.8%), followed by brain metastasis(65.0%), primary lesions(60.0%), and other metastases(53.0%), and there were significant differences(P<0.05). There was a linear correlation between the expression of IL-13Rα2 and the number of MDSCs in cases with liver metastasis(r=0.740, P<0.05). The average survival time of 48 patients with distant metastasis was(12.6±5.2)months. Conclusion The increased level of IL-13Rα2 suggests that the prognosis of colorectal cancer patients with distant metastasis is poor, and it is speculated that IL-13Rα2 can induce proliferation of MDSCs and promote liver metastasis by regulating colorectal cancer cells. -
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